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Novus Biologicals recombinant lc3a
HSp27 does not Interact with either <t>LC3A</t> or LC3B isoforms in the way that it interacts with LC3C. A Far Western approach was implemented. rLC3A or rLC3B were loaded and incubated with 10 ug of rHSp27. Interactions for ( Bi ) LC3A and ( Bii ) LC3B were then detected by probing the rLC3A or rLC3B blot with mouse Anti-Hsp27 antibody.
Recombinant Lc3a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
DSMZ genus amycolatopsis
A core phylogenomic tree based on 1,104 orthologous proteins illustrates the evolutionary relationship between strain MEP2-6 T and its closely related <t>Amycolatopsis</t> species. Streptomyces scabiei 87.22 T was used as the outgroup. The distance matrices between species are shown at the node.
Genus Amycolatopsis, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DSMZ amycolatopsis
Fig. 1. Phylogenetic tree reconstructed using 16S rRNA gene sequences of 78 validly published species described in the genus <t>Amycolatopsis</t> (an additional sequence for Streptomyces viridis BK 199T was taken as an outgroup) using the neighbour-joining method [18]. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [19]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Jukes–Cantor method [20] and are in the units of the number of base substitutions per site. All positions containing gaps and missing data were eliminated. There were 1350 positions in the final dataset. Evolutionary analyses were conducted in mega7 [21]. Asterisks represent common nodes of the three trees (neighbour-joining, maximum-likelihood and maximum- parsimony).
Amycolatopsis, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DSMZ strains amycolatopsis sp
Fig. 1. Phylogenetic tree reconstructed using 16S rRNA gene sequences of 78 validly published species described in the genus <t>Amycolatopsis</t> (an additional sequence for Streptomyces viridis BK 199T was taken as an outgroup) using the neighbour-joining method [18]. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [19]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Jukes–Cantor method [20] and are in the units of the number of base substitutions per site. All positions containing gaps and missing data were eliminated. There were 1350 positions in the final dataset. Evolutionary analyses were conducted in mega7 [21]. Asterisks represent common nodes of the three trees (neighbour-joining, maximum-likelihood and maximum- parsimony).
Strains Amycolatopsis Sp, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


HSp27 does not Interact with either LC3A or LC3B isoforms in the way that it interacts with LC3C. A Far Western approach was implemented. rLC3A or rLC3B were loaded and incubated with 10 ug of rHSp27. Interactions for ( Bi ) LC3A and ( Bii ) LC3B were then detected by probing the rLC3A or rLC3B blot with mouse Anti-Hsp27 antibody.

Journal: bioRxiv

Article Title: Porphyromonas gingivalis activates Heat-Shock-Protein 27 to drive a LC3C-specific probacterial form of select autophagy that is redox sensitive for intracellular bacterial survival in human gingival mucosa

doi: 10.1101/2024.07.01.601539

Figure Lengend Snippet: HSp27 does not Interact with either LC3A or LC3B isoforms in the way that it interacts with LC3C. A Far Western approach was implemented. rLC3A or rLC3B were loaded and incubated with 10 ug of rHSp27. Interactions for ( Bi ) LC3A and ( Bii ) LC3B were then detected by probing the rLC3A or rLC3B blot with mouse Anti-Hsp27 antibody.

Article Snippet: 5 µg of recombinant LC3C (Origene, TP761768), recombinant LC3B (Sino Biological, 14555-H07E), and recombinant LC3A (Novus Biologicals, NBP1-45308-0.1mg) were resolved by SDS-PAGE as previously in Far Western methodologies [ – ].

Techniques: Western Blot, Incubation

Journal: bioRxiv

Article Title: Porphyromonas gingivalis activates Heat-Shock-Protein 27 to drive a LC3C-specific probacterial form of select autophagy that is redox sensitive for intracellular bacterial survival in human gingival mucosa

doi: 10.1101/2024.07.01.601539

Figure Lengend Snippet:

Article Snippet: 5 µg of recombinant LC3C (Origene, TP761768), recombinant LC3B (Sino Biological, 14555-H07E), and recombinant LC3A (Novus Biologicals, NBP1-45308-0.1mg) were resolved by SDS-PAGE as previously in Far Western methodologies [ – ].

Techniques:

A core phylogenomic tree based on 1,104 orthologous proteins illustrates the evolutionary relationship between strain MEP2-6 T and its closely related Amycolatopsis species. Streptomyces scabiei 87.22 T was used as the outgroup. The distance matrices between species are shown at the node.

Journal: Frontiers in Plant Science

Article Title: Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers

doi: 10.3389/fpls.2024.1346574

Figure Lengend Snippet: A core phylogenomic tree based on 1,104 orthologous proteins illustrates the evolutionary relationship between strain MEP2-6 T and its closely related Amycolatopsis species. Streptomyces scabiei 87.22 T was used as the outgroup. The distance matrices between species are shown at the node.

Article Snippet: At the time of writing, the genus Amycolatopsis included 87 species with validly published names ( , https://lpsn.dsmz.de/genus/amycolatopsis ).

Techniques:

Differential colonial appearances of strain MEP2-6 T and its closest type strains grown on ISP 2 agar medium at 30°C for 14 days. Strain: 1, MEP2-6 T ; 2, Amycolatopsis lexingtonensis JCM 12672 T ; 3, A. pretoriensis JCM 12673 T ; 4, A. eburnea TBRC 9315 T .

Journal: Frontiers in Plant Science

Article Title: Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers

doi: 10.3389/fpls.2024.1346574

Figure Lengend Snippet: Differential colonial appearances of strain MEP2-6 T and its closest type strains grown on ISP 2 agar medium at 30°C for 14 days. Strain: 1, MEP2-6 T ; 2, Amycolatopsis lexingtonensis JCM 12672 T ; 3, A. pretoriensis JCM 12673 T ; 4, A. eburnea TBRC 9315 T .

Article Snippet: At the time of writing, the genus Amycolatopsis included 87 species with validly published names ( , https://lpsn.dsmz.de/genus/amycolatopsis ).

Techniques:

ANIb, ANIm, and dDDH values between strain MEP2-6 T and its closest  Amycolatopsis  species.

Journal: Frontiers in Plant Science

Article Title: Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers

doi: 10.3389/fpls.2024.1346574

Figure Lengend Snippet: ANIb, ANIm, and dDDH values between strain MEP2-6 T and its closest Amycolatopsis species.

Article Snippet: At the time of writing, the genus Amycolatopsis included 87 species with validly published names ( , https://lpsn.dsmz.de/genus/amycolatopsis ).

Techniques:

Proteome comparison of strain MEP2-6 T and closely related type strains of the genus Amycolatopsis based on OrthoVenn3. An UpSet plot showed unique and shared orthologous clusters among species. The left horizontal bar chart depicts the number of orthologous clusters per species, whereas the right vertical bar chart illustrates the number of orthologous clusters shared among the species.

Journal: Frontiers in Plant Science

Article Title: Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers

doi: 10.3389/fpls.2024.1346574

Figure Lengend Snippet: Proteome comparison of strain MEP2-6 T and closely related type strains of the genus Amycolatopsis based on OrthoVenn3. An UpSet plot showed unique and shared orthologous clusters among species. The left horizontal bar chart depicts the number of orthologous clusters per species, whereas the right vertical bar chart illustrates the number of orthologous clusters shared among the species.

Article Snippet: At the time of writing, the genus Amycolatopsis included 87 species with validly published names ( , https://lpsn.dsmz.de/genus/amycolatopsis ).

Techniques: Comparison

Predicted compounds encoded by biosynthetic gene cluster families (GCFs) identified in the genomes of strain MEP2-6 T and its closest  Amycolatopsis  species with ≥50% homology with known functions.

Journal: Frontiers in Plant Science

Article Title: Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers

doi: 10.3389/fpls.2024.1346574

Figure Lengend Snippet: Predicted compounds encoded by biosynthetic gene cluster families (GCFs) identified in the genomes of strain MEP2-6 T and its closest Amycolatopsis species with ≥50% homology with known functions.

Article Snippet: At the time of writing, the genus Amycolatopsis included 87 species with validly published names ( , https://lpsn.dsmz.de/genus/amycolatopsis ).

Techniques:

Endophytic colonization of Solanum tuberosum L. cv. Spunta by the Amycolatopsis strain MEP2-6 T . The potato culture before inoculation of strain MEP2-6 T (A) , hyphae with spore masses of strain MEP2-6 T inoculated into the stem node wounding site (B) , the normal potato culture after inoculation of strain MEP2-6 T for five days (C) , attachment of white mycelia of strain MEP2-6 T on the stem node (D) , sections of S. tuberosum cv. Spunta stem epidermal cells invaded by Amycolatopsis MEP2-6 T (E, F) . Scale bar 20 μm. The boxed part of the image is shown as a magnification on the right side of (E) . Red arrows indicate white mycelia in (B, D) and indicate vegetative cells in (E, F) .

Journal: Frontiers in Plant Science

Article Title: Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers

doi: 10.3389/fpls.2024.1346574

Figure Lengend Snippet: Endophytic colonization of Solanum tuberosum L. cv. Spunta by the Amycolatopsis strain MEP2-6 T . The potato culture before inoculation of strain MEP2-6 T (A) , hyphae with spore masses of strain MEP2-6 T inoculated into the stem node wounding site (B) , the normal potato culture after inoculation of strain MEP2-6 T for five days (C) , attachment of white mycelia of strain MEP2-6 T on the stem node (D) , sections of S. tuberosum cv. Spunta stem epidermal cells invaded by Amycolatopsis MEP2-6 T (E, F) . Scale bar 20 μm. The boxed part of the image is shown as a magnification on the right side of (E) . Red arrows indicate white mycelia in (B, D) and indicate vegetative cells in (E, F) .

Article Snippet: At the time of writing, the genus Amycolatopsis included 87 species with validly published names ( , https://lpsn.dsmz.de/genus/amycolatopsis ).

Techniques:

Fig. 1. Phylogenetic tree reconstructed using 16S rRNA gene sequences of 78 validly published species described in the genus Amycolatopsis (an additional sequence for Streptomyces viridis BK 199T was taken as an outgroup) using the neighbour-joining method [18]. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [19]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Jukes–Cantor method [20] and are in the units of the number of base substitutions per site. All positions containing gaps and missing data were eliminated. There were 1350 positions in the final dataset. Evolutionary analyses were conducted in mega7 [21]. Asterisks represent common nodes of the three trees (neighbour-joining, maximum-likelihood and maximum- parsimony).

Journal: International journal of systematic and evolutionary microbiology

Article Title: Genome-based reclassification of Amycolatopsis eurytherma as a later heterotypic synonym of Amycolatopsis thermoflava .

doi: 10.1099/ijsem.0.004642

Figure Lengend Snippet: Fig. 1. Phylogenetic tree reconstructed using 16S rRNA gene sequences of 78 validly published species described in the genus Amycolatopsis (an additional sequence for Streptomyces viridis BK 199T was taken as an outgroup) using the neighbour-joining method [18]. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [19]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Jukes–Cantor method [20] and are in the units of the number of base substitutions per site. All positions containing gaps and missing data were eliminated. There were 1350 positions in the final dataset. Evolutionary analyses were conducted in mega7 [21]. Asterisks represent common nodes of the three trees (neighbour-joining, maximum-likelihood and maximum- parsimony).

Article Snippet: As on 15 March 2020, 78 validly published species of Amycolatopsis were taxonomically characterized by their correct names (https:// lpsn. dsmz. de/ genus/ amycolatopsis).

Techniques: Sequencing

Fig. 2. Heatmap with a dual dendrogram based on the average nucleotide identity (ANI) values of 48 members of the genus Amycolatopsis. The strains were compared using ANIm (MUMmer based ANI) with default parameters. The ANIm value matrix was then plotted as a dual dendrogram using Clustvis (https://biit.cs.ut.ee/clustvis/). A. thermoflava, A. eurytherma and A. methanolica were highly identical based on the ANIm values.

Journal: International journal of systematic and evolutionary microbiology

Article Title: Genome-based reclassification of Amycolatopsis eurytherma as a later heterotypic synonym of Amycolatopsis thermoflava .

doi: 10.1099/ijsem.0.004642

Figure Lengend Snippet: Fig. 2. Heatmap with a dual dendrogram based on the average nucleotide identity (ANI) values of 48 members of the genus Amycolatopsis. The strains were compared using ANIm (MUMmer based ANI) with default parameters. The ANIm value matrix was then plotted as a dual dendrogram using Clustvis (https://biit.cs.ut.ee/clustvis/). A. thermoflava, A. eurytherma and A. methanolica were highly identical based on the ANIm values.

Article Snippet: As on 15 March 2020, 78 validly published species of Amycolatopsis were taxonomically characterized by their correct names (https:// lpsn. dsmz. de/ genus/ amycolatopsis).

Techniques:

Fig. 3. Phylogenomic analysis of Amycolatopsis species (n=48) using core-genome alignments. The alignment was performed using mafft [13] and the tree was reconstructed using the best fit model in iq-tree. An unrooted tree with 1000 bootstrap replicates was visualized using iTOL (https://itol.embl.de/about.cgi).

Journal: International journal of systematic and evolutionary microbiology

Article Title: Genome-based reclassification of Amycolatopsis eurytherma as a later heterotypic synonym of Amycolatopsis thermoflava .

doi: 10.1099/ijsem.0.004642

Figure Lengend Snippet: Fig. 3. Phylogenomic analysis of Amycolatopsis species (n=48) using core-genome alignments. The alignment was performed using mafft [13] and the tree was reconstructed using the best fit model in iq-tree. An unrooted tree with 1000 bootstrap replicates was visualized using iTOL (https://itol.embl.de/about.cgi).

Article Snippet: As on 15 March 2020, 78 validly published species of Amycolatopsis were taxonomically characterized by their correct names (https:// lpsn. dsmz. de/ genus/ amycolatopsis).

Techniques: